diff --git a/eng/targets/Helix.Common.props b/eng/targets/Helix.Common.props
index d68970f8dc..029f75990e 100644
--- a/eng/targets/Helix.Common.props
+++ b/eng/targets/Helix.Common.props
@@ -29,6 +29,7 @@
   <ItemGroup Condition="'$(IsWindowsOnlyTest)' != 'true' AND '$(TargetArchitecture)' == 'x64' AND '$(IsHelixDaily)' == 'true'">
     <HelixAvailableTargetQueue Include="Windows.7.Amd64.Open" Platform="Windows" />
     <HelixAvailableTargetQueue Include="Windows.81.Amd64.Open" Platform="Windows" />
+    <HelixAvailableTargetQueue Include="Windows.10.Amd64.Server20H1.Open" Platform="Windows" />
     <HelixAvailableTargetQueue Include="Ubuntu.1804.Amd64.Open" Platform="Linux" />
     <HelixAvailableTargetQueue Include="Ubuntu.2004.Amd64.Open" Platform="Linux" />
     <HelixAvailableTargetQueue Include="Centos.7.Amd64.Open" Platform="Linux" />
diff --git a/src/Middleware/Diagnostics/test/FunctionalTests/Diagnostics.FunctionalTests.csproj b/src/Middleware/Diagnostics/test/FunctionalTests/Diagnostics.FunctionalTests.csproj
index b7caf9c87e..20ca9cd1dc 100644
--- a/src/Middleware/Diagnostics/test/FunctionalTests/Diagnostics.FunctionalTests.csproj
+++ b/src/Middleware/Diagnostics/test/FunctionalTests/Diagnostics.FunctionalTests.csproj
@@ -5,6 +5,7 @@
     <SignAssembly>false</SignAssembly>
     <AssemblyName>Diagnostics.FunctionalTests</AssemblyName>
     <TestDependsOnMssql>true</TestDependsOnMssql>
+    <SkipHelixQueues>Windows.10.Amd64.Server20H1.Open</SkipHelixQueues>
   </PropertyGroup>
 
   <ItemGroup>