diff --git a/src/ProjectTemplates/Web.ProjectTemplates/BlazorServerWeb-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/BlazorServerWeb-CSharp.csproj.in
index b4020a5d98..868f60d4af 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/BlazorServerWeb-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/BlazorServerWeb-CSharp.csproj.in
@@ -11,7 +11,7 @@
 
   <!--#if (IndividualLocalAuth && !UseLocalDB) -->
   <ItemGroup>
-    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" />
+    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" ExcludeFromSingleFile="true" />
   </ItemGroup>
 
   <!--#endif -->
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/RazorPagesWeb-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/RazorPagesWeb-CSharp.csproj.in
index b23eae2881..ab090ad09e 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/RazorPagesWeb-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/RazorPagesWeb-CSharp.csproj.in
@@ -13,7 +13,7 @@
 
   <!--#endif -->
   <ItemGroup Condition=" '$(IndividualLocalAuth)' == 'True' AND '$(UseLocalDB)' != 'True' ">
-    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" />
+    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" ExcludeFromSingleFile="true" />
   </ItemGroup>
 
   <!--#if (IndividualB2CAuth || IndividualLocalAuth || OrganizationalAuth) -->
diff --git a/src/ProjectTemplates/Web.ProjectTemplates/StarterWeb-CSharp.csproj.in b/src/ProjectTemplates/Web.ProjectTemplates/StarterWeb-CSharp.csproj.in
index a33301f1a2..70d65ede37 100644
--- a/src/ProjectTemplates/Web.ProjectTemplates/StarterWeb-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.ProjectTemplates/StarterWeb-CSharp.csproj.in
@@ -13,7 +13,7 @@
 
   <!--#endif -->
   <ItemGroup Condition=" '$(IndividualLocalAuth)' == 'True' AND '$(UseLocalDB)' != 'True' ">
-    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" />
+    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" ExcludeFromSingleFile="true" />
   </ItemGroup>
 
   <!--#if (IndividualB2CAuth || IndividualLocalAuth || OrganizationalAuth) -->
diff --git a/src/ProjectTemplates/Web.Spa.ProjectTemplates/Angular-CSharp.csproj.in b/src/ProjectTemplates/Web.Spa.ProjectTemplates/Angular-CSharp.csproj.in
index 621db9e5b6..950d707d42 100644
--- a/src/ProjectTemplates/Web.Spa.ProjectTemplates/Angular-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.Spa.ProjectTemplates/Angular-CSharp.csproj.in
@@ -27,7 +27,7 @@
   </ItemGroup>
 
   <ItemGroup Condition=" '$(IndividualLocalAuth)' == 'True' AND '$(UseLocalDB)' != 'True' ">
-    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" />
+    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" ExcludeFromSingleFile="true" />
   </ItemGroup>
   <ItemGroup>
     <!-- Don't publish the SPA source files, but do show them in the project files list -->
diff --git a/src/ProjectTemplates/Web.Spa.ProjectTemplates/React-CSharp.csproj.in b/src/ProjectTemplates/Web.Spa.ProjectTemplates/React-CSharp.csproj.in
index 69925a032f..5682766065 100644
--- a/src/ProjectTemplates/Web.Spa.ProjectTemplates/React-CSharp.csproj.in
+++ b/src/ProjectTemplates/Web.Spa.ProjectTemplates/React-CSharp.csproj.in
@@ -24,7 +24,7 @@
   </ItemGroup>
 
   <ItemGroup Condition=" '$(IndividualLocalAuth)' == 'True' AND '$(UseLocalDB)' != 'True' ">
-    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" />
+    <None Update="app.db" CopyToOutputDirectory="PreserveNewest" ExcludeFromSingleFile="true" />
   </ItemGroup>
   <ItemGroup>
     <!-- Don't publish the SPA source files, but do show them in the project files list -->